Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: The cuproptosis blocker SS-31 ameliorated airway responsiveness and airway inflammation in chronic asthma model mice (n = 10 mice per group). ( A ) Ers, Rrs and Rn in different groups challenged with aerosol Mch (mg/ml). ( B ) Representative images of HE-stained lung tissue. C. Cell classification (eosinophils, lymphocytes, and neutrophils) and counts in BALF. D. The levels of the inflammatory factors IL-4, TGFβ1 and IL-1β in the BALF. The data are shown as the means ± SEMs, n = 10. One-way analysis of variance (ANOVA) and the Student–Newman–Keuls (SNK) method were used, and P < 0.05 was considered to indicate statistical significance. *p < 0.05 for the OVA group vs. the control group. # p < 0.05 for the SS-31 + OVA group vs. the PBS + OVA group. Above experiment was repeated three times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Aerosol, Staining, Control

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Cuproptosis was increased in BEAS-2B cells incubated with rhIL-4 and TGFβ1 and the addition of SS-31 reversed the above process. ( A ) Quantification of the effects of different concentrations of SS-31 on the cell viability of BEAS-2B cells. ( B ) Cu 2+ levels in the supernatant of BEAS-2B cells. ( C ) Cu 2+ levels in the cytoplasm of BEAS-2B cells. D. Protein expression of cuproptosis-related genes (FDX-1, LIAS, and DLAT) in BEAS-2B cells. ( D ) Quantification of the protein expression levels of cuproptosis-related genes in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the SS-31 + rhIL-4 + TGFβ1 group vs. the PBS + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Incubation, Expressing

Journal: Scientific Reports

Article Title: Cuproptosis of bronchial epithelial cells triggers airway remodeling in chronic asthma

doi: 10.1038/s41598-025-34574-3

Figure Lengend Snippet: Silencing FDX-1 attenuated cell remodeling in BEAS-2B cells incubated with rhIL-4 and TGFβ1. ( A , B ) Quantification of FDX-1 protein expression in BEAS-2B cells transfected with different siRNAs. ( C ) Cell morphology in different groups was observed under a light microscope. ( D , E ) Quantification of the effect of FDX-1 knockdown on cell migratory function. F. Effects of FDX-1 knockdown on the protein expression of EMT-related MMP-2, MMP-9, E-cadherin, N-cadherin, vimentin and smooth muscle-related α-SMA in BEAS-2B cells. The data are shown as the means ± SEMs. *p < 0.05 for the rhIL-4 + TGFβ1 PBS group vs. the rhIL-4 + TGFβ1 group. # p < 0.05 for the Scr + rhIL-4 + TGFβ1 group vs. the Si + rhIL-4 + TGFβ1 group. Above experiment was repeated fourth times.

Article Snippet: Cytokine levels (interleukin-4 (IL)-4, TGFβ1 and IL-1β) (Multi Sciences Biotech Co., Ltd) in the BALF of the mice were measured via individual ELISA kits according to the manufacturers’ instructions.

Techniques: Incubation, Expressing, Transfection, Light Microscopy, Knockdown

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits the migration and invasion of A549 and H1299 cells induced by TGFβ1. ( A ) Wound healing experiments. Control group, TGFβ1 group, TGFβ1 + GSK-J4 group were set up respectively, and scratch widths at 0 h, 24 h and 48 h were observed to evaluate cell migration. ( B , C ) Transwell migration experiment. The ability of the cells to pass to the lower chamber was observed. ( D-F ) RT-qPCR and western blot. The mRNA and protein levels of mmp2 /MMP2 and mmp9 /MMP9, which are related to cell migration and invasion were detected. Scale: 200 μm.(Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Migration, Control, Quantitative RT-PCR, Western Blot

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits TGFβ1-induced EMT of A549 and H1299 cell lines. ( A ) RT-qPCR was used to detect EMT related markers, cdh1 , cdh2 , fn1 . ( B , C ) Western blot analysis was performed to detect the expression changes of E-cadherin, N-cadherin and FN1 at the protein levels.(Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: GSK-J4 inhibits TGFβ1-induced EMT of A549 and H1299 cell lines via the Wnt/β-catenin signaling pathway. ( A ) Changes of markers associated with the Wnt/β-catenin signaling pathway at mRNA levels. ( B, C ) Western blot was used to detect the changes of markers related to Wnt/β-catenin signaling pathway at the protein level. ( D )Expression and localization of β-catenin in A549 and H1299 cells induced by TGFβ1 by immunofluorescence staining. Scale bar : 100 μm. (Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1; # TGFβ1 vs. TGFβ1 + GSK-J4)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Control

Journal: Medical Oncology (Northwood, London, England)

Article Title: Anti-tumor effects and mechanism of the histone demethylase inhibitor GSK-J4 in non-small cell lung cancer cells

doi: 10.1007/s12032-025-03185-3

Figure Lengend Snippet: Restoration experiments were performed on Licl treated cells. ( A, B ) Transwell performed cell migration experiments. ( C–F ) Changes in the protein levels of MMP2 and MMP9, markers related to cell migration and invasion. ( G–J ) Western blot detection of changes in markers related to the Wnt/β-catenin signaling pathway at protein level. (Data are means ± SEM from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. * control vs. TGFβ1;#TGFβ1 vs. TGFβ1 + GSK-J4; ^TGFβ1 + GSK-J4 vs. TGFβ1 + GSK-J4 + Licl)

Article Snippet: GSK-J4 (T3100) and TGFβ1 (TMPY-02638) was purchased from Targetmol, Shanghai, China.

Techniques: Migration, Western Blot, Control